The Comparison and Test of Antioxidant Activity of Ethanol Extracts of Pangi Leaves and Seeds (Pangium Edule Reinw.) Using the DPPH (1,1-Diphenyl-2-Picrylhydrazyl) Method
Perbandingan Dan Uji Aktivitas Antioksidan Ekstrak Etanol Daun Dan Biji Pangi (Pangium Edule Reinw.) Dengan Metode DPPH (1,1-Diphenyl-2-Picrylhydrazyl)
Abstract
The leaves and seeds of pangi (Pangium edule Reinw.) contain alkaloids, flavonoids, saponins, and tannins, which acts as antioxidants. Antioxidants are needed to protect the body and inhibit the negative impact of free radicals due to the oxidation reactions that occur. Antioxidants are compounds that inhibit oxidation reactions by binding free radicals and molecules. This research aims to determine the comparison of the antioxidants activity of pangi (Pangium edule Reinw.) leaves and seeds using the DPPH (1,1,-Diphenyl-2-Picrylhydrazyl) method. Reactive research method, experimentally, namely by carrying out serial dilutions of concentrations of 10, 20, 30, 40 and 50 ppm in each test solution, then adding the DPPH solution and measuring the antioxidants activity with a UV-Vis Spectrophotometer. After that, the IC50 (Inhibitor Concentration 50) value and the AAI (Antioxidant Activity Index) value are calculated, and the data were analyzed using ANOVA. The results of calculating the IC50 and AAI values are that the IC50 value of the pangi leaf ethanol extract is 49.6061 ppm (AAI=1.007), while the IC50 value of ethanol extract of pangi seeds is 50.2027 (AAI=0.9959). From the results of research and data analysis, it can be concluded that the ethanol extract of pangi leaves has higher antioxidant activity compared to the ethanol extract of pangi seeds.
References
Astuty Lolo, W., & Yamlean, P. V. (2017). Identifikasi Kandungan Fitokimia dan Uji Kadar Hambat Minimum dan Kadar Bunuh Minimum Ekstrak Etanol Daun Pangi (Pangium edule Reinw.) terhadap Pertumbuhan Bakteri Escherichia coli. Jurnal Ilmiah Farmasi Pharmacon, 6(3), 260-267.
Elmitra, Yenti, R., & Chandra, W. (2022). Formulasi Sediaan Gel dari Ekstrak Kulit Batang Menteng (Baccaurea macrocarpa). Jurnal Akademu Farmasi, 7(1), 1–20.
Handayani, V., Ahmad, A. R., Sudir, M., Etlingera, P., & Sm, R. M. (2017). Uji Aktivitas Antioksidan Ekstrak Metanol Bunga dan Daun Patikala ( Etlingera elatior ( Jack ) R . M . Sm ) Menggunakan Abstrak. Pharm Sci Res, 1(2), 86–93.
Lumempow, F., Yosevina Lumempow, F., Yudistira, A., & Juliana Suoth, E. (2023). Uji Aktivitas Antioksidan Ekstrak Dan Fraksi Spons Stylissa Carteri Yang Diperoleh Dari Pulau Manado Tua. Pharmacy Medical Journal, 6(1).
Mailuhu, M., Runtuwene, M. R. J., & Koleangan, H. S. . (2017). Skrining Fitokimia dan Aktivitas Antioksidan Ekstrak Metanol Kulit batang Soyogik (Saurauia Bracteosa DC.). Jurnal Ilmiah Sains, 10(1), 68.
Sari, R. S., & Suhartati. (2015). Pangi (Pangium Edule Reinw.) Sebagai Tanaman Dan Sumber Pangan. Balai Penelitian Kehutanan Makassar Jl Perintis Kemerdekaan Km, dan, & Selatan.
Purwanto, D., Bahri, S., & Ridhay, A. (2017). Uji Aktivitas Antioksidan Ekstrak Buah Purnajiwa (Kopsia Arborea Blume.) Dengan Berbagai Pelarut. Kovalen, 3(1), 24.
Putra, I. M. A. S., & Erliana, N. K. E. (2022). Pengaruh Ekstrak Etanol Daun Pangi (Pangium edule) terhadap Penyembuhan Luka Bakar pada Tikus Putih (Rattus novergicus) Galur Wistar. Jurnal Ilmiah Medicamento, 8(2), 127–133.
Sakul, G., Simbala, H., & Rundengan, G. (2020). Uji Daya Hambat Ekstrak Etanol Daun Pangi (Pangium edule Reinw. ex Blume) Terhadap Bakteri Staphylococcus aureus, Escherichia coli Dan Pseudomonas aeruginosa The Inhibition Test Of Ethanol Extract Of Pangi (Pangium edule Reinw. ex Blume) Leaves Againts Staphylococcus aureus, Escherichia coli And Pseudomonos aeruginosa.
Wardani, R. K., Putri, Y. M., Nurjanah, D. A., & Susidarti, R. A. (2021). Mengungkap Potensi Kluwak (Pangium edule Reinw) Sebagai Antioksidan Alami dan Penghambat Senescence. Jurnal Ilmu Kefarmasian Indonesia, 19(2), 189.
