Identifikasi Kandungan Formalin Pada Tahu Putih Di Pasar Tradisional Kecamatan Klaten Tengah dengan Metode Spektrofotometri UV-Vis

Alifia Nuraini; Meila Diana; Mitsalina Fildzah Arifah

doi:10.61902/cerata.v16i2.1856

Alifia Nuraini Universitas Jenderal Achmad Yani Yogyakarta
Meila Diana Universitas Jenderal Achmad Yani Yogyakarta
Mitsalina Fildzah Arifah Universitas Jenderal Achmad Yani Yogyakarta

DOI: https://doi.org/10.61902/cerata.v16i2.1856

Keywords: Formalin, Klaten, Tofu, Traditional market, Spectrophotometry UV-Vis

Abstract

White tofu is a high-protein food widely consumed by the public but has a short shelf life due to its high water content. To extend its shelf life, some vendors use formalin as a preservative, although its use is prohibited due to its toxic nature and potential to cause digestive disorders, organ damage, and cancer. Traditional markets in Central Klaten District serve as economic centers for the local community; however, information regarding the presence of formalin in white tofu remains limited. This study aimed to identify presence of formalin in white tofu sold at these markets, qualitatively and quantitatively. A non-experimental descriptive design was used, involving organoleptic testing over three days, qualitative analysis using KMnO₄, Fehling A & B, Schiff, and chromotropic acid reagents, determination of the maximum wavelength, and quantitative analysis using the UV-Vis spectrophotometry method. Signs of spoilage were observed in all samples through organoleptic testing. No colour change was detected in qualitative analysis, and no absorption peak was observed at 580 nm. The validity of the UV-Vis spectrophotometry method was demonstrated by a linearity and linear range with r = 0.999, %Recovery from accuracy of 97.807-101.023%, %RSD from precision were 1.206%, a limit of detection (LOD) of 2.811 ppm, and a limit of quantification (LOQ) of 8.519 ppm. Samples suspected of containing formalin were confirmed to be free of formalin based on the quantitative test. The limitation of this study was its lack of sensitivity toward very low formalin concentrations. In conclusion, formalin was not detected in any sample through organoleptic, qualitative, and quantitative test. The validity of the UV-Vis spectrophotometry method met the required validation parameters.

References

AOAC International. (2016). Appendix F: Guidelines for Standard Method Performance Requirements, Official Methods of Analysis of AOAC INTERNATIONAL. Available at: https://doi.org/10.1093/9780197610145.005.006.
Apriliyani, S. A., Martono, Y., Riyanto, C. A., Mutmainah, M., & Kusmita, L. (2018). Validation of UV-VIS Spectrophotometric Methods for Determination of Inulin Levels from Lesser Yam (Dioscorea esculenta L.). Jurnal Kimia Sains dan Aplikasi, 21(4), pp. 161–165. Available at: https://doi.org/10.14710/jksa.21.4.161-165.
Arifah, F. N., Fitria, F., & Septyaningrum, E. (2024). Analisis Kualitatif dan Kuantitatif Vitamin C pada Buah Ceremai (Phyllanthus acidus (L.) Skeels.). Jurnal Pharma Bhakta, 4(2), pp. 47–54.
Arziyah, D., Yusmita, L., & Ariyetti. (2019). Analisis Mutu Tahu dari Beberapa Produsen Tahu di Kota Padang. Jurnal Teknologi Pertanian Andalas, 23(2), pp. 143–148.
Ayuchecaria, N., Sari, A. K., & Fatmawati, E. (2017). Analisis Kualitatif Formalin pada Ayam yang Dijual di Pasar Lama Wilayah Banjarmasin. Jurnal Ilmiah Ibnu Sina, 2(1), pp. 51–59.
BPOM RI. (2018). Peraturan Badan Pengawas Obat dan Makanan Nomor 7 Tahun 2018 Tentang Bahan Baku yang Dilarang dalam Pangan Olahan. Retrieved March 21, 2025, from https://peraturan.bpk.go.id/Details/183201/peraturan-bpom-no-7-tahun-2018.
Dewi, S. R. (2019). Identifikasi Formalin pada Makanan Menggunakan Ekstrak Kulit Buah Naga. Jurnal Nasional Ilmu Kesehatan (JNIK), 2(1), pp. 45–51.
Djamal, N., Cahyadi, D., & Maulana, Y. A. (2023). Analisis Kualitas Produk Tahu Kuning dengan Metode Six Sigma-Kaizen di PD. TBS. Prosiding Seminar Nasional Penelitian LPPM UMJ. Serang, Banten, pp. 1–9.
Eurachem. (2025). The Fitness for Purpose of Analytical Methods A Laboratory Guide to Method Validation and Related Topics (3rd ed). Europe.
Fernandez, J. P. S., Mendoza, M. D. V, Torre, B. C. B., & Quinto, E. C. (2018). Development of a Detection Method of Formalin in Foods Based on Chromotropic Acid Test. Department of Chemical Engineering, University of Santo Tomas Manila, Philippines.
Fitri, A. S., & Fitriana, Y. A. N. (2020). Analisis Senyawa Kimia pada Karbohidrat. Sainteks, 17(1), pp. 45–52. Available at: https://doi.org/10.30595/sainteks.v17i1.8536.
Fitrianingsih, N., Mustafa, C. H., & Sunyoto, S. (2019). Penetapan Kadar Formalin pada Tahu di Pasar X dengan Metode Spektrofotometri Visible. CERATA Jurnal Ilmu Farmasi, 10(1), pp. 10–17. Available at: https://doi.org/10.61902/cerata.v10i1.69.
Herdhiansyah, D., Reza, R., Sakir, S., & Asriani, A. (2022). Kajian Proses Pengolahan Tahu: Studi Kasus Industri Tahu di Kecamatan Kabangka Kabupaten Muna. Agritech : Jurnal Fakultas Pertanian Universitas Muhammadiyah Purwokerto, 24(2), pp. 231–237. Available at: https://doi.org/10.30595/agritech.v24i2.13375.
Horwitz, W., & Albert, R. (2006). The Horwitz Ratio (HorRat): A Useful Index of Method Performance with Respect to Precision. Journal of AOAC International, 89(4), pp. 1095–1109. Available at: https://doi.org/10.1093/jaoac/89.4.1095.
ICH Harmonised Guideline. (2023). Validation of Analytical Procedures Q2(R2), ICH Harmonisation for Better Helath.
Marliza, H., Suhaera, S., & Saputri, T.A. (2019). Analisis Kualitatif Formalin pada Ikan Asin di Pasar Jodoh Kota Batam. Jurnal Farmasi Indonesia, 16(02), pp. 307–314.
Miasur, M. P., Suhardi, B., & Suletra, I. W. (2021). Pengukuran Pemenuhan Standar GMP dan WISE pada Pabrik Tahu Karya Mukti Bandungan. Performa: Media Ilmiah Teknik Industri, 20(2), pp. 189–198. Available at: https://doi.org/10.20961/performa.20.2.53448.
Muslich, M. A., Isnaeni, I., & Sudjarwo, S. (2020). Validasi Metode Spektrofotometri UV untuk Penetapan Kadar Kolkisin dalam Infus Kembang Sungsang. Berkala Ilmiah Kimia Farmasi, 7(1), pp. 7–13.
Rahmawati, Y.D. (2022). Analisis Kualitatif Formalin pada Tahu yang Beredar di Pasar Desa Kupu Kota Brebes. Jurnal Gizi Aisyah, 5(2), pp. 12–16.
Rosita, N. (2020). Analisis Kandungan Formalin pada Tahu di Pasar Tradisional dan Pasar Swalayan Kota Tangerang Selatan, Pusat Penelitian dan Penerbitan (Puslitpen). LP2M UIN Syarif Hidayatullah Jakarta.
Rosita, N. (2022). Uji Formalin pada Tahu yang Diperdagangkan di Ciputat Tangerang Selatan. Dalton : Jurnal Pendidikan Kimia dan Ilmu Kimia, 5(1), pp. 51–59. Available at: https://doi.org/10.31602/dl.v5i1.6766.
Sinurat, J. P., Karo, R. M. B., & Syarifuddin, S. (2023). Sosialisasi Penyalahgunaan dan Bahaya Formalin Sebagai Pengawet dalam Bahan Makanan di Kecamatan Lubuk Pakam. Jurnal Pengmas Kestra (Jpk), 3(1), pp. 88–93. Available at: https://doi.org/10.35451/jpk.v3i1.1767.
Sulthoniyah, S. T. M. & Rachmawati, N. F. (2022). Identifikasi Kandungan Formalin dan Boraks pada Ikan Asin di Pasar Tradisional Karangrejo Kecamatan Banyuwangi. Jurnal Ilmu Perikanan dan Kelautan, 4(2), pp. 78–83.
Suseno, D. (2021). Validasi Metode Analisis Formalin dan Aplikasinya Pada Ikan Asin. Jurnal Agroindustri Halal, 7(2), pp. 173–182.
Umbingo, S. C., Sudewi, S., & Wewengkang, D. S. (2015). Validasi Metode Analisis Formalin dalam Daging Paha Ayam Di Kota Manado. Pharmacon, 4(3), pp. 139–146.
United State Pharmacopeia (2024) Validation of Compendial Procedures, 1225.
Widianto, W., Sudaryantiningsih, C., & Pambudi, Y. S. (2021). Analisa Cemaran Eschericia Coli dan Salmonella SP serta Kualitas Fisik Tahu ditinjau dari Sanitasi Pabrik Tahu di Sentra Industri Tahu Krajan Mojosongo Surakarta. Intelektiva : Jurnal Ekonomi, Sosial & Humaniora, 03(03), pp. 1–11.