Pengaruh Durasi dan Konsentrasi Pelarut Ekstrak Etanol Daun Teh (Camellia sinensis) Menggunakan Metode Ultrasonik Terhadap Aktivitas Antibakteri Escherichia coli
The Effect of Duration and Concentration of Ethanol Extract Solvent from Tea Leaves (Camellia Sinensis) Using the Ultrasonic Method on the Antibacterial Activity of Escherichia Coli
Keywords:
antibacterial, Ultrasonic Assisted Extraction (UAE), Camellia sinensis, duration, consentration of ethanol
Abstract
Tea leaves (Camellia sinensis) are one of the plants that contain flavonoids, tannins, alkaloids, and secondary metabolites of catechins that have the potential as antibacterials. Extraction using the Ultrasonic Assisted Extraction (UAE) method is influenced by the duration and concentration of the solvent. The purpose of this study was to determine the effect of different durations and solvent concentrations on the antibacterial activity of Escherichia coli tea extract using the UAE method. Tea leaves were extracted using the UAE method with sonication waves using 50%, 70%, and 96% ethanol solvents with a duration of 15, 30, and 45 minutes, as much as 25 grams in 250 ml. The extract was dissolved in 10% DMSO and an antibacterial activity test was carried out with a concentration of 75% as much as 50 µL per well. The negative control in the bacterial activity test used 10% DMSO, and used ciprofloxacin as a positive control. The antibacterial activity test was determined using the well diffusion method to determine the diameter of the inhibition zone. The test results data were analyzed statistically using SPSS. The results showed that tea leaves have antibacterial activity against Escherichia coli bacteria, the resulting yield related to variations in solvent concentration and extraction duration did not differ significantly, however the results of the inhibition test against Escherichia coli bacteria showed significant differences. Tea leaf extract extracted with UAE with 70% ethanol for 30 minutes had the highest inhibition diameter resulting in antibacterial activity with an average value of 13.475mm ± 0,38.
References
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Bria, D.I., Missa, H. and Sombo, I.T. (2022) ‘Isolasi Dan Karakterisasi Bakteri Escherichia coli Pada Bahan Pangan Berbasis Daging Di Kota Kupang PENDAHULUAN Berdasarkan Undang-Undang Republik Indonesia Nomor 7 Tahun 1996 tentang Pangan dan Peraturan Pemerintah Republik Indonesia Nomor 28 Tahun 2004’, Jurnal Sains dan Terapan, 1(2), pp. 82–89.
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Hartanti, A.I., Permana, I.D.G.M. and Puspawati, K.D. (2021) ‘Pengaruh Konsentrasi Etanol pada Metode Ultrasonikasi terhadap Aktivitas Antioksidan Ekstrak Daun Gonda (Sphenoclea zeylanica)’, Jurnal Ilmu dan Teknologi Pangan, 10(2), pp. 163–171.
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Kemenkes RI (2017) Farmakope Herbal Indonesia Edisi II.
Kumar, K., Srivastav, S. and Sharanagat, V.S. (2021) ‘Ultrasound assisted extraction (UAE) of bioactive compounds from fruit and vegetable processing by-products: A review’, Ultrasonics Sonochemistry, 70(September 2020), p. 105325. Available at: https://doi.org/10.1016/j.ultsonch.2020.105325.
Lenggu, C.K.L., Rini, D.I. and Shinta, A.L. (2020) ‘Uji Aktivitas Ekstrak Etanol Kulit Daging Buah Lontar (Borassus Flabellifer Linn) Terhadap Pertumbuhan Escherichia Coli Secara in Virto’, Cendana Medical Journal (CMJ), 19(1), pp. 96–107.
Marlina Kristina, C.V., Ari Yusasrini, N.L. and Yusa, N.M. (2022) ‘Pengaruh Waktu Ekstraksi Dengan Menggunakan Metode Ultrasonic Assisted Extraction (UAE) Terhadap Aktivitas Antioksidan Ekstrak Daun Duwet (Syzygium cumini)’, Jurnal Ilmu dan Teknologi Pangan (ITEPA), 11(1), p. 13. Available at: https://doi.org/10.24843/itepa.2022.v11.i01.p02.
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Oktavia, N. and Pujiyanto, S. (2018) ‘Isolasi dan Uji Antagonisme Bakteri Endofit Tapak Dara ( Catharanthus Roseus , L .) Terhadap Bakteri Escherichia coli dan Staphylococcus aureus’, J. Berkala Bioteknologi, 1(1), pp. 6–12.
Purnama Sari, I., Ulvia, R. and Putra Pratama, N. (2024) ‘Pengaruh Metode Ekstraksi Terhadap Kadar Flavonoid Total (Citrus aurantifolia)’, Jurnal Ilmiah Farmasi Attamru, 5(2), pp. 100–113. Available at: https://journal.uim.ac.id/index.php/Attamru.
Ro Berlina, A.Y., Lumbangaol, N.Y. and Simanjuntak, H.A. (2023) ‘Aktivitas Antibakteri Ekstrak Etanol Kulit Jeruk Nipis (Citrus aurantifolia (Christm) Swingle) Terhadap Staphylococcus aureus’, Herbal Medicine Journal, 6(1), pp. 15–20. Available at: https://doi.org/10.58996/hmj.v6i1.77.
Rusmawijayanto, T., Luliana, S. and Isnindar (2019) ‘PROFIL KROMATOGRAFI LAPIS TIPIS EKSTRAK ETANOL DAUN SENGGANI (Melastoma malabathricum L.) METODE PERKOLASI’, Farmasi Kalbar Universitas Tanjungpura, 4(1).
Safitri, E., Hidayati, N.A. and Hertati, R. (2019) ‘PREVALENSI BAKTERI Salmonella PADA AYAM POTONG YANG DIJUAL DI PASAR TRADISIONAL PANGKALPINANG’, EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi, 4(1), pp. 25–30. Available at: https://doi.org/10.33019/ekotonia.v4i1.1012.
Sari, D. (2013) AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL KULIT BATANG TUMBUHAN SALA (Cynometra ramiflora L) TERHADAP Staphylococcus aureus, Escherichia coli DAN Klebsiella pneumoniae BESERTA BIOAUTOGRAFINYA.
Shaikh, J.R. and Patil, M. (2020) ‘Qualitative tests for preliminary phytochemical screening: An overview’, International Journal of Chemical Studies, 8(2), pp. 603–608. Available at: https://doi.org/10.22271/chemi.2020.v8.i2i.8834.
Syaron, P. et al. (2020) ‘Uji Senyawa Fitokimia dan Aktivitas Antioksidan Tanaman Patah Tulang ( Euphorbia tirucalli L . )’, 9(2), pp. 64–69.
Wardani, T.S. and Tanikolan, R.A. (2021) ‘Analisis Cemaran Bakteri Escherichia Coli, Salmonella pada Depot Amiu Kelurahan Cemani Kabupaten Sukoharjo’, Seminar Informasi Kesehatan Nasional., pp. 148–157. Available at: https://medicra.umsida.ac.id/index.php.
WHO (2024) Diarrhoeal disease, WHO. Available at: https://www.who.int/news-room/fact-sheets/detail/diarrhoeal-disease.
